Abcam, ab14475, Anti-Frizzled 5 antibody

Size: 50µg
Rabbit Polyclonal Frizzled 5 antibody. Suitable for WB, IHC-FoFr, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:ICC/IF, WB, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human FZD5 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q13467,
Specificity:Recognizes human Fz5.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: 30% Glycerol (glycerin, glycerine), 0.609% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Frizzled 5 also known as FZD5 is a receptor that functions mechanically as a part of the Wnt signaling pathway. The Frizzled 5 protein has a molecular weight of around 75 kDa and is expressed in various tissues including the brain the eyes and the cardiovascular system. As a transmembrane receptor Frizzled 5 binds to Wnt ligands and activates intracellular signaling cascades mediating important cellular processes.
Biological function summary
Frizzled 5 participates in cellular communication and is part of the Wnt-frizzled protein complex. This complex plays a role in embryonic development cell proliferation and cell differentiation. Through its interaction with Wnt ligands Frizzled 5 can influence cell fate determination and contribute to the maintenance of stem cells. These activities are essential for development and tissue homeostasis.
Pathways
Knowledge of Frizzled 5 places it within both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway Frizzled 5 associates with LRP5/6 co-receptors to stabilize β-catenin leading to gene transcription changes. In the non-canonical pathway it involves the activation of Ca2+ signaling. Proteins related to Frizzled 5 in these pathways include Dishevelled (DVL) and the Glycogen synthase kinase (GSK-3).
Research has linked Frizzled 5 to conditions like retinopathy of prematurity and colorectal cancer. In retinopathy Frizzled 5's interaction with Norrin—a protein related to eye vascular development—can lead to abnormal vascularization. In colorectal cancer alterations in the Wnt-Frizzled signaling are implicated with β-catenin accumulating as a consequence. The modification in these signaling pathways hints at the potential for therapeutic interventions targeting Frizzled 5.