Abcam, ab14613, Anti-PDE4D antibody

Size: 50µg
Rabbit Polyclonal PDE4D antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 25 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab14613 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9 or PDE4C. The antibody has not been tested against endogenous PDE4D, only recombinant proteins and we therefore cannot guarantee it will detect endogenous PDE4.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphodiesterase 4D (PDE4D) is an enzyme that hydrolyzes cyclic AMP (cAMP) to its inactive form AMP controlling important cellular signaling pathways. It is part of the large PDE family and sometimes referred to as DPDE1. With a molecular weight of approximately 93 kDa PDE4D is widely expressed in the brain heart lungs and immune cells. Its expression in these tissues indicates an important regulatory role in both central and peripheral physiological processes.
Biological function summary
PDE4D plays an important role in the modulation of intracellular cAMP levels impacting processes such as inflammation cognition and cardiac function. It does not operate as part of a larger complex but collaborates with other signaling molecules to influence cellular responses. PDE4D's regulation of cAMP levels directly affects protein kinase A (PKA) leading to alterations in gene expression and cell activity.
Pathways
PDE4D integrates into the cAMP signaling and inflammatory pathways impacting various cellular responses. In the cAMP signaling pathway it partners with other PDEs and signaling proteins such as PKA which phosphorylates target proteins affecting cellular function. In inflammatory pathways PDE4D modulates immune cell activity by altering the cAMP-mediated inhibition of pro-inflammatory cytokine production linking it to inflammation-related responses.
PDE4D has associations with conditions such as asthma and ischemic stroke. In asthma PDE4D regulates cAMP levels in lung cells affecting bronchial constriction and inflammation. In ischemic stroke alterations in PDE4D activity influence vascular function by changing cAMP dynamics in vascular smooth muscle cells. Within these disorders PDE4D interacts with proteins like cytokines and COX-2 which also play parts in inflammatory pathways and vascular responses.