Abcam, ab14686, Anti-SGLT1 antibody

Size: 100µg
Anti-SGLT1 antibody (ab14686) is a rabbit polyclonal antibody detecting SGLT1 in Western Blot, IP, ELISA, Dot Blot . Suitable for Human, Rat . - Over 60 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat,
Applications:Dot, ELISA, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SLC5A1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.P13866

Product details:
What is this antibody validated in?
Anti-SGLT1 antibody (ab14686) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ELISA, Dot Blot in Human, Rat samples.
What is the molecular weight of SGLT1?
Anti-SGLT1 (ab14686) specifically detects a band for SGLT1 (UniProt: P13866) at a molecular weight of 73kDa.
Trusted by the scientific community
Anti-SGLT1 (ab14686) was first used in a scientific publication in 2004 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-SGLT1 (ab14686) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified on antigen sepharose affinity column, Storage buffer-Preservative: 0.02% Sodium azideConstituents: HEPES, Tris glycine, 30% Glycerol (glycerin, glycerine), 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SGLT1 also known as sodium/glucose cotransporter 1 or SLC5A1 is a membrane protein actively responsible for glucose and galactose uptake in the small intestine and renal tubules. It has a molecular mass of approximately 73 kDa. Expressed mainly in the brush border of the enterocytes in the small intestinal lining and to a lesser degree in the kidney SGLT1 functions by coupling the transport of glucose to sodium ions' electrochemical gradient enabling effective nutrient absorption.
Biological function summary
SGLT1's main role is in facilitating nutrient absorption in the gastrointestinal tract. It operates as part of the larger SGLT protein family but does not form a complex with other proteins. The protein is essential for the transport of monosaccharides from the gut into circulation. By ensuring glucose and galactose absorption during digestive processes SGLT1 provides necessary carbohydrates for cellular metabolism and systemic energy homeostasis.
Pathways
The transport protein SGLT1 is integral to the glucose uptake pathway. This pathway intersects with the insulin signaling pathway because the glucose absorbed by SGLT1 later enters systemic circulation. SGLT1 also complements the GLUT family of transporters especially GLUT2 which function downstream by facilitating glucose transport across the intestinal basolateral membrane into the bloodstream. The interplay between these transport mechanisms maintains glucose levels post-ingestion.
SGLT1 dysfunction is linked to glucose-galactose malabsorption a genetic disorder causing severe dehydration and diarrhea due to glucose and galactose accumulation in the lumen. Additionally research investigates SGLT1's contribution to diabetes management given its role in glucose absorption. The protein's activity along with related proteins like GLUT2 and GLUT4 could influence insulin resistance and glucose homeostasis in metabolic syndromes. Understanding SGLT1 in these contexts enables potential therapeutic interventions for conditions involving disordered glucose metabolism.