Abcam, ab14713, Anti-NDUFA9 antibody [20C11B11B11]

Size: 100µg
Anti-NDUFA9 antibody [20C11B11B11] (ab14713) is a mouse monoclonal antibody detecting NDUFA9 in Western Blot, Flow Cytometry, IHC-P . Suitable for Cow, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 240 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:20C11B11B11,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human,
Applications:IHC-P, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-NDUFA9 antibody [20C11B11B11] (ab14713) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Cow, Human, Mouse, Rat samples.
What is the molecular weight of NDUFA9?
Anti-NDUFA9 [20C11B11B11] (ab14713) specifically detects a band for NDUFA9 (UniProt: Q16795) at a molecular weight of 40kDa.
Trusted by the scientific community
Anti-NDUFA9 [20C11B11B11] (ab14713) was first used in a scientific publication in 2004 and has been cited over 240 times in peer-reviewed journals.
Reviewed by scientists
Anti-NDUFA9 [20C11B11B11] (ab14713) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-NDUFA9 antibody [20C11B11B11] (ab14713) has been confirmed by Immunocytochemistry testing in NDUFA9 Knockout HAP1 cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.4 - 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NDUFA9 also known as NADH: ubiquinone oxidoreductase subunit A9 plays a mechanical role as a core component of Complex I in the mitochondrial respiratory chain. This protein facilitates the transfer of electrons from NADH to ubiquinone an essential step in cellular energy production. NDUFA9 has a molecular weight of about 39 kDa and is expressed in tissues with high energy demands like the heart and brain.
Biological function summary
NDUFA9 functions as an important element of the mitochondrial respiratory complex I contributing to ATP production through oxidative phosphorylation. It is part of the multi-subunit enzyme Complex I which consists of other integral subunits such as NDUFS1 and NDUFV2. These elements work synergistically for efficient electron transport and proton pumping across the mitochondrial inner membrane.
Pathways
NDUFA9 integrates into essential pathways like the electron transport chain and oxidative phosphorylation pathway. It works closely with proteins such as NDUFS3 and NDUFB8 essential for the integrity and activity of Complex I. The electron transport chain plays a significant role in generating the proton-motive force necessary for ATP synthesis directly linking NDUFA9 to energy metabolism.
Dysfunction of NDUFA9 relates to mitochondrial disorders like Leigh syndrome and Parkinson's disease. Mutations or deficiencies in the NDUFA9 protein can impair electron transport and ATP production leading to cellular energy deficits. In Leigh syndrome other proteins such as ND3 and COX1 may be involved while in Parkinson's disease interactions with proteins like alpha-synuclein and PINK1 have been reported.