Abcam, ab14928, Anti-Bestrophin/BEST1 antibody

Size: 50µg
Rabbit Polyclonal Bestrophin/BEST1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BEST1 aa 400-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human BEST1 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.O76090

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bestrophin also known as BEST1 or VMD2 is a protein that functions as a calcium-activated chloride channel. It plays an important role in the transport of ions across cell membranes. The molecular mass of BEST1 is about 68 kDa. This protein is prominently expressed in the retinal pigment epithelium (RPE) which is a layer of cells in the eye vital to the function and survival of the photoreceptors. These photoreceptors are responsible for capturing light and initiating vision.
Biological function summary
BEST1 influences the regulation of ionic homeostasis in the eye and contributes to the RPE's maintenance. It acts as part of a chloride channel complex that helps transport chloride ions which is important for fluid absorption and secretion in the retina. There are interactions with calcium ions that activate the channel allowing it to regulate the flow of other ions in response to changes in calcium concentration which is essential for maintaining the ionic balance necessary for proper vision.
Pathways
BEST1 plays significant roles in the visual cycle and ion transport pathways in the retina. It is related to the protein ANO2 another chloride channel although they interact differently with calcium ions. BEST1 influences the fluid regulation within the retina an essential part of the visual cycle pathway. It signals the movement of ions in response to external stimuli maintaining the balance of fluids and ions that reh cells function correctly.
Mutations in the BEST1 gene are connected to Best vitelliform macular dystrophy and adult-onset foveomacular dystrophy. Both disorders lead to retinal degeneration affecting vision. The interaction between BEST1 and other chloride channels like ANO2 suggests possible effects on the severity and progression of these retinal diseases highlighting the need for continued research into therapeutic interventions and the complex network of protein interactions within the retina.