Abcam, ab150344, Anti-Leishmania A2 antibody [C9]

Size: 50µg
Mouse Monoclonal Leishmania A2 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Leishmania samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:C9,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Leishmania,
Applications:IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. It is specific to A2 protein from Leishmania donovani and in a highly probable manner to other species which express A2. It won't be detected in species where the A2 is a non-expressed pseudogene such as Leishmania major.ab150344 recognizes the family of A2 proteins ranging from 42 to 100kDa.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Lyophilized protein G purified in PBS pH7.4, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Leishmania A2 also known as LmA2 is a protein expressed in Leishmania parasites. It plays an important role in the stress response mechanisms of these protozoan organisms especially under heat shock conditions. The mass of Leishmania A2 is approximately 45 kDa. Expression of Leishmania A2 takes place predominantly in the amastigote form of the parasite which is the stage responsible for causing disease in mammalian hosts.
Biological function summary
The functions of Leishmania A2 involve helping the parasite survive within the hostile environment of the host's macrophages. This protein is not known to be part of a larger protein complex but functions to enhance parasite virulence. By assisting in the adaptation to harsh temperature changes from insect vectors to mammalian hosts it aids in protecting intracellular amastigotes from heat shock-induced damage.
Pathways
Leishmania A2 functions within pathways that facilitate stress response and host-parasite interactions. It is intricately involved with the mechanisms that regulate heat shock protein expression and immune evasion. It associates with heat shock proteins such as Hsp70 which work together to stabilize other proteins and refold denatured proteins. This interaction helps the parasite to cope with thermal and oxidative stresses encountered upon entering the human host.
Leishmania A2 is intimately connected to the pathogenesis of leishmaniasis particularly the visceral form of this disease. This protein contributes to the parasite's ability to persist and multiply within host macrophages evading immune detection and response. Additionally its interactions with host immune regulators such as Hsp90 highlight its role in modulating host immune pathways during infection. Understanding Leishmania A2 can provide insights into developing therapies to target leishmaniasis by disrupting its stress response and survival mechanisms.