Abcam, ab152211, Recombinant Human ATP5F1 protein

Size: 10µg
Recombinant Human ATP5F1 protein is a Human Full Length protein, in the 1 to 256 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P24539,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5F1 also known as ATP synthase F(0) complex subunit B1 is a protein found in the inner mitochondrial membrane. It is a component of the ATP synthase enzyme an important player in energy production with a molecular weight around 55 kDa. ATP5F1 is expressed abundantly in energy-demanding tissues such as the heart liver and skeletal muscle. This protein plays an important mechanical role in synthesizing adenosine triphosphate (ATP) by catalyzing the combination of adenosine diphosphate (ADP) and inorganic phosphate.
Biological function summary
ATP5F1 serves as an important part of the ATP synthase complex which consists of various subunits forming the F1 and Fo regions. This complex carries out the final step of oxidative phosphorylation in the mitochondria by using the proton gradient across the mitochondrial membrane to drive ATP production. As a critical process in cellular respiration ATP generation influences numerous cellular functions including muscle contraction nerve impulse propagation and synthesis of biomolecules.
Pathways
ATP5F1 operates within the oxidative phosphorylation and electron transport chain pathways. These pathways are important for maintaining the cell's energy balance. ATP5F1 works closely with other proteins such as ATP synthase Fo subunits aiding in proton translocation and ensuring efficient ATP synthesis. Disruptions in these pathways can have significant effects on cellular metabolism and energy homeostasis.
ATP5F1 is linked to mitochondrial dysfunctions such as mitochondrial myopathy and Leigh syndrome. These conditions result from impaired oxidative phosphorylation leading to insufficient ATP production. ATP5F1 interacts with the NADH dehydrogenase complex and mutations can affect their functionality contributing to these disorders. Understanding ATP5F1's role helps researchers explore therapeutic strategies aimed at managing these mitochondrial diseases.