Abcam, ab153973, Anti-GLUD1 antibody

Size: 100µL
Rabbit Polyclonal GLUD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Drosophila melanogaster samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLUD1 aa 50-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Drosophila melanogaster, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GLUD1 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.P00367,
Specificity:Due to high sequence homology with GLUD2 there is a potential for cross-reactivity.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLUD1 also known as glutamate dehydrogenase 1 is an enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and ammonia playing an important role in amino acid metabolism. The molecular mass of GLUD1 is approximately 61 kDa. It is expressed in many tissues with notably high levels in liver kidney and neural tissues. GLUD1 is essential for maintaining the balance between amino acid and carbohydrate metabolism.
Biological function summary
GLUD1 facilitates nitrogen and carbon homeostasis through its role in amino acid breakdown and energy metabolism. Although not part of a complex GLUD1's activity impacts central metabolic processes influencing cellular energy status and neurotransmitter regulation. The enzyme contributes to the glutamate-glutamine cycle which is important for neurotransmission in the brain. Its regulation by ADP GDP and other molecules enables cells to adapt to energetic demands.
Pathways
GLUD1 is integral to the tricarboxylic acid (TCA) cycle and urea cycle impacting both energy production and detoxification of ammonia. In the TCA cycle GLUD1 influences the availability of α-ketoglutarate interfacing with enzymes such as isocitrate dehydrogenase. In the urea cycle it aids in converting ammonia to urea in the liver. Additionally GLUD1's function connects with glutamine synthetase in managing amino acid synthesis and degradation.
GLUD1 is associated with congenital hyperinsulinism and hepatic encephalopathy. Hyperinsulinism often results from mutations in GLUD1 which lead to excessive insulin release due to increased leucine sensitivity. In hepatic encephalopathy GLUD1's role in ammonia detoxification becomes important with impaired function potentially leading to elevated ammonia levels and neurological dysfunction. Understanding GLUD1's interactions with other proteins such as those in insulin signaling pathways could further elucidate its impact on these medical conditions.