Abcam, ab154088, Anti-HADH antibody

Size: 100µL
Rabbit Polyclonal HADH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HADH aa 50 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human HADH aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q16836

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HADH protein also known as 3-hydroxyacyl-CoA dehydrogenase facilitates the penultimate step in the beta-oxidation of fatty acids. It catalyzes the conversion of 3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme exhibits a molecular mass of about 34 kDa. HADH is expressed predominantly in the liver kidney heart and muscle tissues reflecting its role in energy metabolism.
Biological function summary
The HADH enzyme holds significant responsibility for mitochondrial fatty acid oxidation. It plays a role in the degradation of medium and short-chain fatty acids. As part of a larger enzyme complex it works integrally with other dehydrogenases to maintain cellular energy levels and metabolic homeostasis especially during fasting or periods of increased energy demand.
Pathways
HADH functions prominently in the beta-oxidation pathway of fatty acid metabolism. This enzyme works in conjunction with acyl-CoA dehydrogenases further highlighting its role in breaking down fatty acids into acetyl-CoA an important molecule for energy production in the citric acid cycle. The activity of HADH interacts closely with other mitochondrial enzymes to maintain efficient energy turnover.
Mutations in the HADH gene contribute significantly to diseases such as hyperinsulinism and mitochondrial trifunctional protein deficiency. These conditions showcase the importance of HADH in metabolic regulation and energy balance. HADH-related dysfunctions also connect with enzymes like short-chain 3-hydroxyacyl-CoA dehydrogenase evidencing its role in broader metabolic disease contexts.