Abcam, ab154530, Anti-EPLIN antibody

Size: 100µL
Rabbit Polyclonal EPLIN antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIMA1 aa 450-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human LIMA1 aa 450-750. The exact immunogen used to generate this antibody is proprietary information.Q9UHB6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EPLIN also known as Epithelial Protein Lost In Neoplasm is an actin-associated protein with significant roles in cell structure and function. It has a molecular mass of approximately 100 kDa. EPLIN exists in two isoforms: EPLIN-α and EPLIN-β with different expression patterns and functions. Various tissues express EPLIN with a high concentration seen in epithelial cells. EPLIN associates closely with the cytoskeletal framework stabilizing actin filaments and contributing to cellular architecture and motility.
Biological function summary
EPLIN plays a critical role in the regulation of cell dynamics. It operates as a structural component by binding to filamentous actin enhancing its stability and promoting bundled formations. It also suppresses actin filament depolymerization supporting cell adhesion and movement. As part of actin-associated complexes EPLIN interacts with proteins involved in cell signaling pathways such as RhoA influencing actin-binding properties and cellular responses. Its presence in these complexes implies a broader role in maintaining cellular integrity and mechanical support.
Pathways
Several elements illustrate how EPLIN integrates into signaling cascades. The actin cytoskeleton remodeling pathway prominently features EPLIN. Its interaction with RhoA and other GTPase family members controls actin filament dynamics influencing changes in cell shape and migration. The Wnt signaling pathway also involves EPLIN where it modulates actin cytoskeleton stability affecting cellular proliferation and differentiation. These pathways illustrate how EPLIN coordinates with proteins such as RhoA to facilitate critical cellular functions.
EPLIN exhibits associations with conditions such as cancer and fibrosis. In cancer reduced EPLIN expression correlates with increased cell migration and invasion contributing to tumor progression and metastasis. This relationship positions EPLIN as a potential biomarker for cancerous transformations. In fibrosis aberrant EPLIN expression affects cellular contractility and extracellular matrix composition implicating it in disease pathology. Connections with cancer-related proteins like E-cadherin and β-catenin further emphasize EPLIN's role in disease mechanisms influencing epithelial-mesenchymal transitions and cellular adhesion dynamics.