Abcam, ab154661, Anti-DIP13B/APPL2 antibody

Size: 100µL
Rabbit Polyclonal DIP13B/APPL2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human APPL2 aa 450 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human APPL2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8NEU8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DIP13B also known as APPL2 is a protein with significant roles in cellular processes. It has an approximate mass of 85 kDa and is expressed in various tissues including the brain and heart. Mechanically APPL2 interacts with components of the cell signaling machinery influencing endosomal trafficking. The protein also participates in signal transduction pathways by regulating receptor-mediated endocytosis.
Biological function summary
APPL2 functions as a critical player in cellular signaling and homeostasis. It forms part of signaling complexes that include proteins like Akt and DNML1 where it modulates their activities. APPL2 contributes to cell survival proliferation and metabolism by impacting pathways connected to insulin signaling and other growth factor receptors. Its regulatory ability extends to the fine-tuning of cellular responses to extracellular cues.
Pathways
APPL2 plays integral roles in the PI3K/Akt signaling pathway and the neurotrophic signaling pathway. In both pathways it associates with Akt an important kinase in ensuring cells respond adequately to growth signals and stress. APPL2's influence on the PI3K/Akt pathway affects cellular metabolism growth and survival ensuring that cells maintain proper function under various conditions. By engaging with neurotrophic signaling APPL2 affects neuronal survival and plasticity making it important for neural function and development.
APPL2 has connections with insulin resistance and certain neurological disorders. In the context of insulin resistance its interaction with Akt suggests a role in metabolic dysfunctions where impaired APPL2 function might contribute to the pathogenesis. In neurological disorders changes in APPL2 levels may influence conditions such as Alzheimer's disease where altered Akt signaling is present. The relationship between APPL2 and other proteins like the insulin receptor highlights its involvement in both metabolic and neurological disease states.