Abcam, ab154856, Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

Size: 20µL / 100µL / 250µL / 1mL
Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is a rabbit monoclonal antibody detecting VDAC1/Porin in Western Blot, IHC-P, IHC-Fr, ICC/IF, Flow Cytometry (Intra) . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10852(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) and Flow Cytometry (Intra) in Human, Mouse, Rat samples.
What is the molecular weight of VDAC1/Porin?
Anti-VDAC1/Porin + VDAC2 [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is often used as a loading control in Western blot. It specifically detects a band for VDAC1/Porin (UniProt: P21796) at a molecular weight of 31kDa.
Trusted by the scientific community
Anti-VDAC1/Porin + VDAC2 [EPR10852(B)] - Mitochondrial Loading Control (ab154856) was first used in a scientific publication in 2012 and has been cited over 90 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) has been confirmed by Western blot testing in VDAC1 Knockout HAP1 cell line,
ab255444
Other related products
We have a range of other formats of antibody clone [EPR10852(B)] also available for your convenience: ab154856, HRP -
ab185063
, Carrier free -
ab240128
, PE -
ab317896
, APC -
ab317934
, Alexa Fluor® 488 -
ab317974
, Alexa Fluor® 594 -
ab318015
, Alexa Fluor® 555 -
ab318057
, Alexa Fluor® 647 -
ab318099
, Alexa Fluor® 750 -
ab321135
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The VDAC1/Porin and VDAC2 proteins also known as voltage-dependent anion-selective channel 1 and 2 serve as important pore-forming proteins within the mitochondrial outer membrane. Each VDAC protein has a molecular mass of about 30-35 kDa. Commonly referred to as the 'porin hub' due to their central role in metabolite exchange VDAC1 and VDAC2 facilitate the transport of ions and small molecules between the mitochondria and cytoplasm regulating energy production and cellular metabolism. They express widely across various tissues highlighting their essential role in cellular functions.
Biological function summary
Voltage-dependent anion channels like VDAC1/Porin and VDAC2 modulate the exchange of ions and metabolic substrates playing a significant role in apoptosis and the maintenance of mitochondrial functions. As integral parts of mitochondrial complexes they interact with proteins such as hexokinase which anchors to VDAC1 and influences glycolytic flux linking energy production with apoptotic signaling pathways. This positions VDAC proteins as key regulators of cellular energy homeostasis.
Pathways
VDAC1/Porin and VDAC2 engage prominently in the apoptotic and mitochondrial permeability transition pathways. In the apoptosis pathway they interact with anti-apoptotic proteins like Bcl-xl and pro-apoptotic members like Bax highlighting their dual role in cell survival and death. These interactions highlight the proteins' contribution to mitochondrial outer membrane permeabilization (MOMP) a critical event in the release of cytochrome c and subsequent apoptosome formation further integrating them into broader signaling networks.
Dysfunctions in VDAC1/Porin and VDAC2 link to neurodegenerative diseases and cancer. Aberrant VDAC1 activity connects with Alzheimer's disease due to its role in mitochondrial dysfunction affecting amyloid-beta peptide accumulation and neuronal cell death. In cancer VDAC overexpression associates with altered cellular metabolism and resistance to apoptosis positioning these proteins as potential therapeutic targets. Interactions with proteins such as hexokinase and Bcl-2 family members further emphasize their role in these pathological processes.