Abcam, ab155449, Mouse MIP-1 gamma ELISA Kit (CCL9)

Size: 1 x 96Tests
Mouse MIP-1 gamma ELISA Kit (CCL9) is a Sandwich (quantitative) ELISA for the measurement of Mouse MIP-1 gamma (CCL9) in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 1 pg/mL,
Range:1 - 250 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's MIP-1 (CCL9) gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MIP-1 gamma in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP-1 (CCL9 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Macrophage Inflammatory Protein 1 gamma (MIP-1γ) also known as CCL9 functions as a chemokine involved in immune cell signaling. It weighs approximately 11 kDa. MIP-1γ attracts various immune cells to sites of inflammation within the body. The protein is expressed mainly in macrophages but you can also find it in other immune cells like dendritic cells. It plays a significant role in the body's response to inflammatory stimuli by mediating the recruitment of leukocytes.
Biological function summary
MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.
Pathways
MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.
MIP-1γ connects to various conditions including chronic inflammatory diseases and certain cancers. For example it has a link to rheumatoid arthritis where it contributes to the persistence of inflammation by recruiting immune cells to affected joints. It is also associated with some types of leukemia where its expression levels can influence disease progression. Proteins like interleukin-1β interact with MIP-1γ in these conditions as they both participate in inflammatory responses that exacerbate disease states.