Abcam, ab155450, Mouse Sonic Hedgehog-N ELISA Kit

Size: 1 x 96Tests
Mouse Sonic Hedgehog-N ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Sonic Hedgehog-N in Mouse in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell Lysate,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 5 pg/mL,
Range:5 - 1200 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's Shh-N Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Shh-N in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse Shh-N coated on a 96-well plate. Standards and samples are pipetted into the wells and Shh-N present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Shh-N antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Shh-N bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sonic Hedgehog N also known as Shh is a signaling molecule that plays an important role in cell communication during embryonic development. It is a protein with a mass of approximately 19 kDa. In mammals including mice Shh is mainly expressed in the developing central nervous system limbs and certain regions of the gastrointestinal tract. This molecule is locally secreted and operates by interacting with receptor proteins on the surface of target cells.
Biological function summary
Sonic Hedgehog N regulates the growth and patterning of tissues. It acts by binding to the Patched receptor part of the Hedgehog signaling complex which subsequently releases inhibition on another protein called Smoothened. This interaction facilitates the transcription of target genes involved in cell differentiation and tissue organization. Shh is also essential for the maintenance of stem cell populations in adult tissues.
Pathways
Sonic Hedgehog N is a central player in the Hedgehog signaling pathway which is both an important regulator of embryonic development and a fine tuner of adult homeostasis. The pathway interacts with proteins such as Gli which are transcription factors modulating gene expression downstream of Shh signaling. Shh also interfaces with the Wnt signaling pathway frequently contributing to coordinated control of cell proliferation and differentiation.
Sonic Hedgehog N has significant implications in cancers like basal cell carcinoma and medulloblastoma. Aberrant activation of the Shh pathway can lead to uncontrolled cell growth and tumor formation. Additionally defects in Shh signaling are associated with congenital disorders like holoprosencephaly where the structure of the brain is affected. Proteins like PTCH1 and SUFU which regulate or are regulated by Shh are also important to understanding its role in these diseases.