Abcam, ab155691, Anti-Cytohesin 3 antibody

Size: 100µL
Rabbit Polyclonal Cytohesin 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYTH3 aa 1-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CYTH3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.O43739

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytohesin 3 also known as CYTH3 is a small GTPase regulator involved in cellular signal transduction. It weighs approximately 47 kDa and is primarily expressed in brain tissues and immune cells. CYTH3 functions as a guanine nucleotide exchange factor (GEF) for Arf family of small GTPases. Through its Sec7 domain it facilitates the exchange of GDP for GTP activating Arf proteins. Its pleckstrin homology (PH) domain aids in membrane association by binding phosphoinositides therefore localizing CYTH3 activity to cell membranes where signal transduction events occur.
Biological function summary
Signaling activities that require rapid membrane dynamics involve CYTH3. It participates in vesicular trafficking cell adhesion and cytoskeletal rearrangements. CYTH3 plays a role in processes such as endocytosis and exocytosis due to its interaction with Arf proteins involved in these pathways. Additionally it contributes to immune responses and cellular communication by being part of signaling complexes that respond to extracellular stimuli.
Pathways
CYTH3 acts as an important regulator in the Arf signaling cascade and also the PI3K/Akt pathway. In the Arf signaling pathway CYTH3 modulates vesicle formation and membrane trafficking. In the PI3K/Akt pathway CYTH3 interacts with phosphoinositides and impacts downstream signaling processes linked to cell survival growth and metabolism. Related proteins include Arf6 which functions in endocytosis and PI3K which is involved in many cellular pathways related to growth and survival.
The dysregulation of CYTH3 activity can contribute to the pathology of neurodegenerative diseases and immune system dysfunctions. Abnormal CYTH3 activity in neurons can affect synaptic vesicle trafficking which is related to diseases like Alzheimer’s. Additionally improper regulation in immune cells may shift cellular responses contributing to inflammation and autoimmune diseases. CYTH3's interactions with proteins such as Arf6 and PI3K emphasize its potential role in these conditions.