Abcam, ab155699, Recombinant human TNF alpha protein (Active)

Size: 100µg
Recombinant human TNF alpha protein (Active) is a Human Full Length protein in the 77 to 233 aa range with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA and Functional studies.The predicted molecular weight of ab155699 protein is 17.4 kDa.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 0.1 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:SDS-PAGE, ELISA, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Bioactivity is measured by its binding ability in a functional ELISA.Immobilized ab155699 at 2 μg/mL ( 100 μl/well ) can bind Humira with a linear range of 0.2-2 ng/mL.,
Accession:P01375,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute with sterile deionized water to a concentration of 200 µg/ml.,
Storage buffer:pH: 7.4Constituents: PBS, 5% Trehalose

Product details:
Ensure the validity of your result using our bioactive recombinant human TNF alpha ab155699 as a control in SDS-PAGE.
Analyze your human TNF alpha ELISA data using the ab155699 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE
Check out our ELISA protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)