Abcam, ab15580, Anti-Ki67 antibody

Size: 100µg
Anti-Ki67 antibody (ab15580) is a rabbit polyclonal antibody detecting Ki67 in IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Over 4210 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Product details:
Anti-Ki67 antibody (ab15580) is a rabbit polyclonal antibody and is validated for use in ICC/IF and IHC-P.
Anti-Ki67 antibody (ab15580) was first used in a scientific publication in 1970 and has been cited over 4218 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Ki67 antibody (ab15580) has high sensitivity and specificity.
The specificity of Anti-Ki67 antibody (ab15580) has been confirmed by ICC/IF testing in Ki67 knockout HAP1 cells.
Anti-Ki67 antibody (ab15580) has 223 independent reviews from customers.
Anti-Ki67 antibody (ab15580) specifically detects Ki67 (UniProt ID: P46013; Molecular weight: 359kDa) and is sold in 100ug selling sizes.
Ki-67 is a protein marker that indicates cell proliferation, with higher levels indicating more aggressive and rapidly growing tumors. It is used in cancer diagnostics to assess tumor behaviour, guide treatment decisions and predict prognosis based on the rate of tumor cell division.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.