Abcam, ab157095, Anti-RGNEF antibody

Size: 100µg
Rabbit Polyclonal RGNEF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGEF28 aa 1650 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARHGEF28 aa 1650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8N1W1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RGNEF also known as Rho Guanine Nucleotide Exchange Factor functions as a signaling protein with a role in regulating the actin cytoskeleton and cellular architecture. It acts mechanistically by promoting the exchange of GDP for GTP on Rho proteins which are small GTPases involved in cytoskeletal dynamics. The protein has an approximate mass of 218 kDa. RGNEF is widely expressed in many tissues with higher levels found in the brain which suggests an importance in neural structure and function.
Biological function summary
RGNEF serves as a critical part of cell signaling processes that control morphogenesis motility and cell division. It acts within a multi-protein complex that includes other exchange factors and scaffold proteins coordinating its action and enhancing signal specificity. Its activity impacts various cellular functions often intersecting with pathways that regulate cytoskeletal reorganization and neural communication highlighting its importance in maintaining normal cell behavior.
Pathways
The functionality of RGNEF integrates into the Rho GTPase cycle and the MAPK signaling pathway. Through these pathways RGNEF interacts with proteins like RhoA Rac1 and Cdc42 which play pivotal roles in transmitting external signals that modulate the cytoskeleton and gene expression. These interactions help cells respond to environmental cues and sustain their structural integrity influencing developmental and physiological processes.
RGNEF is relevant to neurodegenerative diseases especially Amyotrophic Lateral Sclerosis (ALS) and cancer. Mutations and alterations in RGNEF are linked with the pathogenesis of ALS where its interaction with other proteins like TDP-43 contributes to neuronal dysfunction. In cancer dysregulation of RGNEF signaling is observed implicating its role in tumor progression and metastasis through its association with oncogenic proteins such as RhoA. Understanding the role of RGNEF in these conditions may offer insights into therapeutic strategies.