Abcam, ab157318, PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

Size: 50Test
Mouse Monoclonal CD28 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD28 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CD28.2,
Isotype:IgG1,
Conjugation:PerCP/Cy5.5®,
Excitation/Emission:Ex: 482nm, Em: 695nm,
Carrier free:No,
Reacts with:Human, Primates,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.P10747

Product details:
Purified antibody is conjugated with tandem dye PerCP/Cy5.5
under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary
CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.
Pathways
CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.
CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.