Abcam, ab157808, Anti-Talin antibody [8D4]

Size: 100µL
Mouse Monoclonal Talin 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Frog, Chicken, Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Chicken TLN1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8D4,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Chicken, Human, Frog,
Applications:Flow Cyt (Intra), IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Chicken TLN1.P54939,
Epitope:ab157808 recognizes an epitope within the rod domain of Talin 1.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Talin 2 and Talin 1 are closely related proteins that play important roles in cellular mechanics. Talin 1 sometimes referred to as TLN1 has a molecular mass of around 270 kDa. Talin 2 often called TLN2 shares structural and functional similarities with Talin 1. Both proteins serve as essential components of the focal adhesion complexes that connect integrins to the actin cytoskeleton. These proteins are widely expressed across various tissues with Talin 1 being more abundant in most cell types while Talin 2 shows more specific expression in muscle tissues.
Biological function summary
Talin proteins support cellular adhesion and migration by mediating integrin activation. Talin 1 and Talin 2 bind to integrins and recruit other proteins to form a multi-molecular complex. This interaction facilitates the connection between the extracellular matrix and the intracellular actin cytoskeleton allowing cells to respond to mechanical cues and move towards the desired direction. These functions are especially important in muscle contraction and cell motility processes.
Pathways
Talin proteins actively participate in the integrin signaling pathways. Within this pathway Talin interacts with key proteins such as vinculin and kindlin to modulate cellular adhesion. The integrin signaling pathway is important for various cellular processes including survival differentiation and migration. Through the actin cytoskeleton Talin-associated pathways also connect to the Rho GTPase signaling which regulates cytoskeletal dynamics.
Altered expression or function of Talin proteins associates with various pathological conditions including cancer and muscular dystrophy. Talin 1 has implications in tumor progression through its role in enhancing cell migration and invasion essential processes in metastasis. In muscular dystrophy mutations or dysregulation of Talin 2 can affect muscle integrity and function contributing to disease pathogenesis. Both proteins interact with integrin and kindlin forming part of the complex network implicated in these disorders.