Abcam, ab159127, Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein

Size: 10µg
Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein is a Human Fragment protein, in the 959 to 1068 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P42336,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI 3 kinase catalytic subunit alpha also known as PIK3CA or PI3K-alpha is a protein kinase with significant roles in cellular signaling. This protein has a mass of approximately 124 kDa and is part of the PI3 kinase family. PIK3CA is primarily expressed in various tissues including the liver breast and pancreas where it plays an important role in the regulation of cell growth and survival. The structure of PI3 kinase involves a complex of p110 catalytic subunits and p85 regulatory subunits which facilitate its enzymatic functions.
Biological function summary
The PIK3CA protein phosphorylates the inositol ring of phosphoinositides generating phosphatidylinositol (345)-trisphosphate (PIP3) a lipid second messenger involved in numerous signaling pathways. PIK3CA often acts within a complex interacting with regulatory subunits such as p85 to control its activity. The complex formation enhances its stability and localization to the cell membrane where it can exert its enzymatic actions effectively. In its biological capacity PIK3CA plays a role in cellular proliferation survival and metabolism.
Pathways
The PI3K/Akt signaling pathway involves PIK3CA as an important mediator. This pathway is integral to numerous cellular processes including metabolism growth and survival. PIK3CA links closely with Akt a downstream protein kinase that propagates the signal initiated by PIP3 generation. The mTOR pathway is another critical route involving PIK3CA where it regulates translation and cellular growth. These pathways emphasize the importance of PIK3CA in maintaining cellular homeostasis and responding to external growth signals.
Mutations and aberrations in PIK3CA associate with several cancers especially breast and colorectal cancers. The altered activity of PIK3CA can cause uncontrolled cell proliferation due to constant activation of the PI3K/Akt signaling pathway. This disrupts normal cellular apoptosis and fosters tumor growth. Additionally PIK3CA mutations are linked to certain genetic disorders such as PIK3CA-related overgrowth spectrum (PROS) where overactivation alters normal tissue growth and development. The interaction of PIK3CA with proteins like PTEN a tumor suppressor involved in cancer pathways also highlights its significance in oncogenic processes.