Abcam, ab160522, Recombinant Human ADAMTS2/NPI protein

Size: 10µg
Recombinant Human ADAMTS2/NPI protein is a Human Fragment protein, in the 1112 to 1210 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O95450,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as ADAMTS2.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADAMTS2 also known as NPI is an important enzyme in the ADAMTS family that plays a mechanical role by processing collagen precursors. It specifically removes the N-terminal propeptides from type I and II procollagens which is an essential step in collagen maturation. This protein with a mass of approximately 168 kDa is expressed in tissues rich in collagens such as skin tendons and ligaments. ADAMTS2 has significant activity in the extracellular space where collagen fibril formation occurs.
Biological function summary
The protein functions as a procollagen N-proteinase responsible for collagen synthesis and maturation. The protein is not part of a complex but it interacts directly with collagen molecules to perform its enzymatic activity. By removing propeptides from procollagens ADAMTS2 enables the formation of mature collagen fibrils contributing to the structural integrity of connective tissue.
Pathways
ADAMTS2 is actively involved in the collagen biosynthesis and modifying enzymes pathway. It works closely with the transforming growth factor beta (TGF-β) signaling pathway which regulates fibrosis and wound healing processes. Proteins related through these pathways include tissue inhibitors of metalloproteinases (TIMPs) and other members of the ADAMTS family which together coordinate collagen turnover and tissue remodeling.
The function of ADAMTS2 connects strongly to skin disorders such as Ehlers-Danlos syndrome type VIIC where collagen maturation defects occur. Its impaired activity leads to abnormal connective tissue formation and skin fragility. Additionally scleroderma an autoimmune disorder characterized by fibrosis relates to ADAMTS2 malfunction as abnormal collagen deposition is a central feature of tissue pathology. ADAMTS2’s interactions with its substrates and other metalloproteinases highlight its role in these conditions.