Abcam, ab16289, Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker

Size: 100µL
Mouse Monoclonal TRA-1-81 antibody. Stem Cell marker. Suitable for WB and reacts with Human samples. Cited in 49 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:TRA-1-81,
Isotype:IgM,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
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Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRA-1-81 also known as podocalyxin-like protein 1 (PODXL) or Gp200 is a cell surface protein with a mass of approximately 140 kDa. It belongs to a group of proteins recognized by the SSEA-4 antibody. TRA-1-81 expresses significantly in embryonal carcinoma cells pluripotent stem cells and certain tumors and it has been identified on human embryonic stem cells. This protein acts as a marker for undifferentiated cells due to its presence on the surface playing a role in maintaining the pluripotent state.
Biological function summary
TRA-1-81 functions in maintaining cell pluripotency and influences cell adhesion properties. It does not form part of a larger protein complex but interacts closely with glycans present on the cell surface. These glycans are significant for the protein’s role in cell signaling and maintaining stemness. TRA-1-81's expression level often correlates with the ability of cells to self-renew and resist differentiation highlighting its role in early embryonic development.
Pathways
TRA-1-81 is involved in the regulation of signaling pathways that control cell pluripotency and differentiation notably the Wnt and Hedgehog pathways. These pathways facilitate communication within cells that decide cell fate proliferation and differentiation. TRA-1-81 has relation to other pluripotency-related proteins such as OCT4 and SOX2 through these biological pathways contributing to the complex regulation of stem cell maintenance and development.
TRA-1-81 is involved in various cancers specifically testicular and ovarian cancers. The abnormal expression of this protein indicates cancer stem cells which contribute to tumor growth and resistance to therapies. Additionally TRA-1-81 is connected to metastasis in these cancers parallel to its role in cell adhesion. Its relationship with proteins like E-cadherin in cancer pathways further shows how TRA-1-81 can impact disease progression and resistance mechanisms in tumor environments.