Abcam, ab16659, Anti-Synaptophysin antibody [SP11] - Synaptic Marker

Size: 100µL / 500µL / 1mL
Anti-Synaptophysin antibody [SP11] (ab16659) is a rabbit monoclonal antibody detecting Synaptophysin in Western Blot, IHC-P, IHC-Fr . Suitable for Human, Rat . - Over 20 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP11,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC, WB, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:C-terminus

Product details:
What is this antibody validated in?
Anti-Synaptophysin antibody [SP11] (ab16659) is a rabbit monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Rat samples.
What is the molecular weight of Synaptophysin?
Anti-Synaptophysin [SP11] (ab16659) specifically detects a band for Synaptophysin (UniProt: P08247) at a molecular weight of 38kDa.
Collaboration
Anti-Synaptophysin [SP11] (ab16659) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Trusted by the scientific community
Anti-Synaptophysin [SP11] (ab16659) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.5Preservative: 0.1% Sodium azideConstituents: Tissue culture supernatant, Tris buffered saline, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.
Biological function summary
Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.
Pathways
Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.
Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.