Abcam, ab16660, Anti-Estrogen Receptor alpha antibody [SP1]

Size: 20µL / 100µL / 500µL / 1mL
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) is a rabbit monoclonal antibody detecting Estrogen Receptor alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, mIHC . Suitable for Human . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 60 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP1,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), mIHC, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:C-terminal

Product details:
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC in human samples.
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) has been cited over 62 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) has 7 independent reviews from customers.
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) specifically detects Estrogen Receptor alpha (UniProt ID: P03372; Molecular weight: 66kDa) and is sold in 100 uL, 500 uL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone SP1 - ab18726.
Antibody clone SP1 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 568, Alexa Fluor® 75 (
ab267512
ab282199
ab282651
ab35384
ab35385
ab35386
ab38794
ab312515
ab321152
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Estrogen Receptor alpha (ERα), also known as ESR1, is a nuclear hormone receptor that binds estrogens and is expressed in about 70% of breast cancers. It plays a critical role in tumor development and progression by promoting cell division and growth when activated by estrogen.
This product has switched from a hybridoma to recombinant production format on 21
May 2020.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Estrogen Receptor alpha (ER alpha) also known by the gene symbol ESR1 and the antibody clone names 6F11 is a nuclear hormone receptor with a molecular weight of approximately 66 kDa. It functions in the regulation of gene expression by binding to estrogen response elements (EREs) within DNA. This receptor is expressed in various tissues including breast ovary uterus and brain. ER alpha plays an important role in the physiological effects of estrogens impacting the development and maintenance of female reproductive tissues and secondary sexual characteristics.
Biological function summary
Estrogen Receptor alpha functions as part of a large transcriptional complex. Upon binding estrogen it undergoes a conformational change that allows interaction with other transcriptional regulators like ER SP1. This interaction facilitates the recruitment of coactivators or corepressors influencing the transcription of target genes involved in proliferation differentiation and cell survival. It is critical in mediating estrogen signaling pathways that contribute to normal development and function of female reproductive organs as well as cardiovascular and skeletal systems.
Pathways
Estrogen Receptor alpha engages in estrogen signaling and PI3K/AKT pathways. In estrogen signaling ER alpha interacts with proteins such as AP1 enhancing transcriptional activity related to cell growth and survival. Within the PI3K/AKT pathway ER alpha coordinates with proteins like SRC promoting cell proliferation and survival. Its actions in these pathways impact a wide variety of cellular processes influencing tissue homeostasis and growth regulation.
Estrogen Receptor alpha is linked to breast cancer and osteoporosis. In breast cancer ER alpha expression is a significant factor in tumor development and progression often determining the response to hormonal treatments like tamoxifen. It collaborates with proteins like HER2 in some breast cancer subtypes affecting disease outcomes. In osteoporosis altered estrogen signaling through ER alpha leads to reduced bone density highlighting its important role in maintaining bone health and its potential as a therapeutic target.