Abcam, ab167190, Anti-Spartan antibody

Size: 50µg
Mouse Polyclonal Spartan antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPRTN.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human SPRTN.Q9H040

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Spartan also known as SPRTN (DNA Sequence Nonspecific) is a metalloprotease enzyme weighing approximately 53 kDa. It is an important player in genome maintenance and DNA repair especially in the stress response to DNA replication impediments. SPRTN is widely expressed in various tissues with particularly high expression in proliferative tissues where DNA replication is important such as the liver and bone marrow. Its main mechanical role involves recognizing and processing DNA-protein crosslinks that may hinder replication and transcription processes.
Biological function summary
SPRTN is important for DNA replication fidelity and cellular response to DNA damage. It achieves this by being part of the DNA-protein crosslink repair pathway where it cooperates with other proteins to maintain genomic stability. This function ensures replication continues correctly and prevents harmful mutations. SPRTN operates as a protease to cleave proteins trapped on DNA thereby removing obstacles and allowing replication machinery to advance. This activity demonstrates its vital role in safeguarding genetic information.
Pathways
SPRTN integrates into the DNA damage response pathways such as the Fanconi Anemia pathway coordinating with other proteins to maintain genomic integrity. Additionally SPRTN associates with the homologous recombination repair pathway where it interacts with proteins like RAD51 and BRCA1 to facilitate effective DNA repair. These pathways are essential for managing DNA damage events to minimize the risk of genomic instabilities that may lead to disorders.
SPRTN mutations have strong links to a rare genetic disorder known as Ruijs-Aalfs Syndrome characterized by progeroid features and cancer predisposition due to accumulated DNA damage. This connection highlights its role in maintaining genomic stability. Moreover deficiency in SPRTN has relevance to cancer development as its impaired function leads to compromised DNA repair allowing mutations to accumulate. The loss of this protease's activity can correlate with destabilization of genomic maintenance partners like FANCD2 potentially contributing to oncogenesis.