Abcam, ab167424, Anti-PHKG2 antibody [EPR9727]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PHKG2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9727,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHKG2 also known as phosphorylase kinase gamma 2 is a catalytic subunit of the phosphorylase kinase (PhK) enzyme. PHKG2 has a molecular weight of approximately 44 kDa. This enzyme is responsible for the phosphorylation of serine residues in glycogen phosphorylase which is necessary for glycogen breakdown. PHKG2 is expressed mainly in muscle and liver tissues where it plays a role in the regulation of glycogen metabolism.
Biological function summary
Phosphorylase kinase involving PHKG2 is critical for the regulation of glycogenolysis. It forms a complex with other subunits including alpha beta and delta contributing to the full enzymatic activity of PhK. As part of this multi-subunit complex PHKG2 facilitates the conversion of glycogen to glucose-1-phosphate ensuring that energy production meets cellular demands during physical activity or fasting.
Pathways
The glycogenolysis process involving PHKG2 is tightly linked with the cAMP-dependent protein kinase (PKA) signaling pathway. Phosphorylase kinase gets activated by PKA under hormonal control prompting glycogen breakdown and maintaining glucose homeostasis. Additionally PHKG2's activity is connected with the insulin signaling pathway which integrates with its function to regulate carbohydrate metabolism especially relating to its interaction with proteins such as glycogen synthase and protein phosphatase 1.
Mutations in PHKG2 associate with Glycogen Storage Disease Type IX (GSD IX) a disorder characterized by an inability to properly break down glycogen leading to muscle weakness and hepatomegaly. PHKG2's interaction with proteins like alpha-glucosidase also links it to conditions like Pompe disease where glycogen buildup occurs due to deficient enzyme activity. Monitoring and understanding PHKG2's role in these contexts is important for developing therapeutic strategies.