Abcam, ab168352, Anti-GLUD1 + GLUD2 antibody [EPR11370]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal GLUD1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11370,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLUD1 and GLUD2 also known as glutamate dehydrogenases are enzymes that play roles in amino acid metabolism. GLUD1 enzyme weighs approximately 56 kDa. These enzymes convert glutamate to α-ketoglutarate and ammonia thereby regulating amino acid turnover and nitrogen metabolism. GLUD1 and GLUD2 are expressed in mitochondria across numerous tissues but are highly represented in the liver and brain tissues. Mouse GLUD1 is often studied to understand these functions through model organisms.
Biological function summary
These enzymes participate in critical processes like neurotransmitter regulation and energy production. GLUD1 and GLUD2 provide ammonia and α-ketoglutarate necessary for the Krebs cycle connecting amino acid catabolism to energy production. Although not typically part of a larger complex their activity modulates key metabolic pathways essential for cellular energy.
Pathways
GLUD1 and GLUD2 play central roles in nitrogen and tricarboxylic acid (TCA) cycles. Within these cycles they facilitate interactions with proteins like AMPK linking amino acid metabolism to cellular energy sensing. They influence energy homeostasis by modulating NADH and NADPH production further integrating with metabolic control pathways like gluconeogenesis.
GLUD1 and GLUD2 have associations with hyperinsulinism/hyperammonemia syndrome and neurodegenerative disorders such as Alzheimer’s disease. In the context of hyperinsulinism faulty regulation of GLUD1 can lead to excessive insulin production. In neurodegenerative conditions these proteins by altering neurotransmitter levels possibly interact with proteins such as amyloid-β contributing to disease pathology.