Abcam, ab168549, Human Apolipoprotein CII ELISA Kit

Size: 1 x 96Tests
Human Apolipoprotein CII ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Apolipoprotein CII in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 7 ng/mL,
Range:0.016 - 4 µg/mL,
Assay time:4h

Product details:
Abcam's Apolipoprotein CII Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein CII concentrations in plasma and serum.
An Apolipoprotein CII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein CII also known as ApoC-II is an essential player in lipid metabolism. It is a 79 amino acid protein with an approximate mass of 8.9 kDa. ApoC-II is primarily expressed in the liver and secreted into the bloodstream where it associates with very-low-density lipoproteins (VLDLs) and chylomicrons. This protein serves an important mechanical role as an activator of lipoprotein lipase (LPL) an enzyme that hydrolyzes triglycerides into free fatty acids facilitating their uptake by tissues for energy production or storage.
Biological function summary
ApoC-II plays a critical role in the metabolism of plasma triglycerides. As an important component of the lipoprotein complex it is necessary for effective lipid transport and triglyceride regulation. By activating lipoprotein lipase ApoC-II ensures efficient breakdown of triglycerides in the bloodstream aiding in maintaining lipid homeostasis. Without ApoC-II LPL lacks full activity leading to impaired triglyceride clearance and dyslipidemia.
Pathways
ApoC-II is integral to lipid metabolic pathways particularly in the catabolism of triglyceride-rich lipoproteins. It participates significantly in the lipid digestion pathway where it functions in concert with proteins like ApoE and ApoB which further assist in lipid binding and transport. ApoC-II's regulatory actions within these pathways are important for preventing the build-up of triglycerides in circulation contributing to a balance in energy storage and metabolism.
Mutations or deficiencies in ApoC-II function can lead to hyperlipoproteinemia type Ib a condition characterized by elevated plasma triglycerides and risk of pancreatitis. This disorder manifests due to defective activation of lipoprotein lipase highlighting the target's role alongside proteins such as LPL in lipid clearance. Additionally ApoC-II interactions with cholesterol transport proteins may influence atherosclerosis a condition where plaque builds up in arteries posing further cardiovascular risks.