Abcam, ab168844, Anti-Exportin-5 antibody

Size: 100µg
Rabbit Polyclonal Exportin-5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human XPO5 aa 1150 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human XPO5 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9HAV4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab168844 was affinity purified using an epitope specific to Exportin-5 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Exportin-5 also known as Exp5 and eXportin-5 serves a mechanical role in the cellular export of specific RNA molecules from the nucleus to the cytoplasm. This protein belongs to the karyopherin family and has an approximate molecular mass of about 130 kDa. Exportin-5 primarily expresses in cell types where RNA processing and regulation are actively important though specific distribution patterns vary across different tissues.
Biological function summary
Exportin-5 functions by specifically recognizing and binding to pre-microRNA and small nuclear RNA facilitating their transport out of the nucleus. It operates as a part of a complex with RanGTP which ensures that the RNA cargo is correctly recognized and loaded for transport. This export process is essential for the subsequent RNA maturation and ultimately influences gene expression regulation.
Pathways
While Exportin-5 plays a pivotal role in the RNA transport pathway it is also intricately linked to the microRNA biogenesis pathway. Within these pathways Exportin-5 works closely with RanGTP to mediate the nuclear export of pre-microRNAs setting the stage for their maturation into functional microRNAs in the cytoplasm. It also relates to proteins involved in the RNA-induced silencing complex (RISC) assembly as mature microRNAs are integral parts of RISC.
Exportin-5 appears to be linked to tumorigenesis and certain viral infections. Abnormalities in its function may disrupt microRNA export which can lead to dysregulation of gene expression and contribute to cancer development. Furthermore associations with proteins such as Dicer and RISC-related factors highlight its role in antiviral responses where the microRNA pathway can be modulated by viral mechanisms to evade host defenses.