Abcam, ab169755, Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)]

Size: 20µL / 100µL / 1mL
Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) is a rabbit monoclonal antibody detecting 5 Lipoxygenase/5-LO in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP6072(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat samples.
What is the molecular weight of 5 Lipoxygenase/5-LO?
Anti-5 Lipoxygenase/5-LO [EP6072(2)] (ab169755) specifically detects a band for 5 Lipoxygenase/5-LO (UniProt: P09917) at a molecular weight of 78kDa.
Trusted by the scientific community
Anti-5 Lipoxygenase/5-LO [EP6072(2)] (ab169755) was first used in a scientific publication in 2013 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
5-Lipoxygenase also known as 5-LO or 5-LOX is an enzyme that catalyzes the oxidation of arachidonic acid to produce leukotrienes which are signaling molecules in the body. 5-LOX has a molecular weight of approximately 78 kDa. It is mainly expressed in leukocytes like macrophages neutrophils and mast cells. This enzyme plays an important role in the inflammatory response and the regulation of immune functions in these cells.
Biological function summary
The activity of 5-Lipoxygenase contributes to the production of leukotrienes which are pivotal in mediating inflammatory and allergic responses. This enzyme operates alone but it often interacts with 5-LOX-activating protein (FLAP) to facilitate the synthesis of leukotrienes efficiently. These interactions suggest that 5-LOX has a significant role in cellular processes related to inflammation and immune reactions.
Pathways
5-Lipoxygenase is integral to the arachidonic acid metabolic pathway initiating the biosynthesis of leukotrienes from arachidonic acid. This pathway also involves other enzymes like phospholipase A2 which releases arachidonic acid from membrane phospholipids and cyclooxygenase enzymes (COX) which convert arachidonic acid into prostaglandins. The end products of this pathway including leukotrienes manage and resolve inflammation and immune responses illustrating the collaborative effort between these enzymes.
5-Lipoxygenase is significantly linked to inflammatory diseases such as asthma and atherosclerosis. In asthma the overproduction of leukotrienes due to enhanced 5-LOX activity results in bronchoconstriction and inflammation of the airways. In atherosclerosis leukotrienes contribute to the development and progression of plaques driving chronic inflammation in the vascular system. Additionally through its pathway its activity relates closely to cyclooxygenase enzymes showing its importance in disease mechanisms targeted by anti-inflammatory therapies.