Abcam, ab170244, Mouse Factor D ELISA Kit

Size: 1 x 96Tests
Mouse Factor D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Factor D in Mouse in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 17 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:4h

Product details:
Abcam's Factor D Mouse
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor D in cell culture supernatants, urine, plasma and serum.
A Factor D specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor D specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor D captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.
Biological function summary
Factor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.
Pathways
Factor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.
Abnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.