Abcam, ab170931, Anti-TUBB2A + TUBB2B antibody [EPR11843]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal TBB2A antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11843,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, IP, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TUBB2A and TUBB2B proteins also known as tubulin beta-2A and beta-2B chains are components of microtubules. These proteins have a molecular mass of approximately 50 kDa each and play roles in the cytoskeleton of cells. TUBB2A and TUBB2B are expressed in various tissues with notable expression in the brain. They participate in the dynamic assembly and disassembly of microtubules which are essential for many cellular processes including maintaining cell shape enabling intracellular transport and facilitating cell division.
Biological function summary
TUBB2A and TUBB2B contribute to the formation of the microtubule network that supports cellular functions. These proteins are integral parts of the cytoskeletal complex interacting with alpha-tubulin to form tubulin dimers. This dimeric structure then polymerizes to build microtubules. Their involvement in mitosis helps ensure proper chromosome segregation which is vital for the accurate transmission of genetic information during cell division. TUBB2A and TUBB2B also support neurite outgrowth and are important for neuronal development and function.
Pathways
The TUBB2A and TUBB2B proteins are essential in the mitotic spindle assembly contributing to the spindle checkpoint pathway. This pathway ensures that chromosomes are correctly aligned and attached to spindle fibers before anaphase begins. They also participate in the regulation of the MAPK/ERK signaling pathway which is important for cell growth and differentiation. Related proteins in these pathways include MAD2 which monitors the spindle assembly checkpoint and RAF a kinase in the MAPK pathway.
Mutations in TUBB2A and TUBB2B have links to cortical dysplasia and polymicrogyria which are brain malformations characterized by disorganized brain tissue. These conditions can lead to developmental delays seizures and cognitive impairments. Additionally the TUBB2A and TUBB2B are related to tau a microtubule-associated protein which when altered relates to neurodegenerative diseases like Alzheimer's. The abnormal function of tau proteins can disrupt microtubule stability and lead to cell death in neurons.