Abcam, ab171015, Human Prekallikrein and Kallikrein ELISA Kit

Size: 1 x 96Tests
Human Prekallikrein and Kallikrein ELISA Kit is a sandwich ELISA designed to quantify Human Prekallikrein and Kallikrein with a sensitivity of 0.54 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.54 ng/mL,
Assay time:4h

Product details:
Human Prekallikrein and Kallikrein ELISA Kit ab171015 is a sandwich ELISA to measure Human Prekallikrein and Kallikrein in serum, plasma, cerebral spinal fluid (CSF), saliva, milk with a sensitivity of 0.43 ng/ml.
How the assay works
A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Prekallikrein and Kallikrein ELISA Kit ab171015 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin Peroxidase Conjugate. Incubate at room temperature.
4. Add Chromogen Substrate to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.
Biological function summary
Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.
Pathways
Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.
Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.