Abcam, ab171333, Mouse IL-27 p28 ELISA Kit

Size: 1 x 96Tests
Mouse IL-27 p28 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-27 p28 in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:15.6 - 1000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's mouse IL-27 p28
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse IL-27 p28 in cell culture supernatants, serum and plasma (heparin, EDTA).
An IL-27 p28 specific rat has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for IL-27 p28 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse IL-27 p28 amount of sample captured in plate.
Get better reproducibility in only 90 minutes with Mouse IL-27a ELISA Kit (Interleukin 27 p28 subunit) (
ab206306
) from our SimpleStep ELISA
range.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C