Abcam, ab171575, Human Glucose-6-Phosphate Isomerase ELISA Kit

Size: 1 x 96Tests
Human Glucose-6-Phosphate Isomerase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Glucose-6-Phosphate Isomerase with a sensitivity of 61 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Tissue Homogenate, Serum, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 61 pg/mL,
Range:0.156 - 10 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human Glucose-6-phosphate isomerase ELISA kit (ab171575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glucose-6-phosphate isomerase protein in human cell and tissue extracts, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Glucose-6-phosphate isomerase with 61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Glucose-6-phosphate isomerase (GPI) is a glycolytic enzyme. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis and is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, resulting in immediate neonatal death and neurological impairment.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucose-6-phosphate isomerase also known as phosphoglucose isomerase or phosphohexose isomerase is an enzyme that plays a role in the catalytic conversion of glucose-6-phosphate to fructose-6-phosphate. It weighs approximately 63 kDa and functions in the cytoplasm of many cell types. This enzyme is expressed in various tissues including liver muscle and brain supporting essential cellular processes like glycolysis and gluconeogenesis.
Biological function summary
Glucose-6-phosphate isomerase facilitates the reversible isomerization step in glycolysis and gluconeogenesis pathways. It does not form part of a complex but acts independently to regulate the equilibrium between glucose-6-phosphate and fructose-6-phosphate. This regulation is important in cellular energy homeostasis isomerase activity ensuring proper functioning of these metabolic pathways.
Pathways
Glucose-6-phosphate isomerase participates in both glycolysis and the Calvin cycle. In glycolysis it links with important proteins such as phosphofructokinase and aldolase which continue the glycolytic pathway. The isomerase activity within the pathways ensures the proper balance of the substrates needed for energy production and carbon skeleton rearrangements in cells.
Glucose-6-phosphate isomerase has connections to conditions like nonspherocytic hemolytic anemia and rheumatoid arthritis. In the context of nonspherocytic hemolytic anemia its deficiency impairs normal red blood cell function leading to premature destruction. The enzyme can also act as a cytokine known as neuroleukin related to rheumatoid arthritis through pathways involving immune system interactions. In this disease glucose-6-phosphate isomerase’s altered regulation may affect inflammation and joint health linking it with inflammatory proteins such as tumor necrosis factor (TNF).