Abcam, ab172637, Anti-RAG1 antibody [EPRAGR1]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal RAG1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPRAGR1,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAG1 also known as Recombination Activating Gene 1 is an enzyme that has a mass of approximately 120 kDa. It functions mechanically by initiating the V(D)J recombination process that is essential for the development of immunoglobulin and T-cell receptor gene rearrangement. RAG1 achieves this by introducing double-stranded breaks in DNA guiding the recombination events. This protein is mainly expressed in developing lymphocytes within the bone marrow and thymus playing a significant function in the adaptive immune response.
Biological function summary
The recombination process performed by RAG1 is integral to generating the diversity of antibodies and T-cell receptors which form the foundation of the adaptive immune system. RAG1 acts as part of a protein complex with RAG2 where it collaborates to recognize and bind to recombination signal sequences in the DNA. This complex facilitates the accurate cleavage necessary for recombination enabling lymphocytes to produce a wide variety of receptors capable of recognizing numerous antigens.
Pathways
RAG1 directly participates in the antigen receptor rearrangement pathway which is fundamental to the adaptive immune system. It is closely linked to the DNA repair mechanisms required for joining the broken DNA ends it creates. Key proteins involved in these pathways include RAG2 which forms a complex with RAG1 and the DNA-dependent protein kinase which mediates the repair of DNA double-strand breaks ensuring genomic stability during immune cell development.
Improper function or mutations in RAG1 can result in severe combined immunodeficiency (SCID) or Omenn syndrome both of which severely impair the immune system. RAG1 mutations affect the recombination efficiency leading to decreased production of functional lymphocytes. The disorder connections extend to RAG2 as they together form the active RAG complex and defects in either can disrupt immune function highlighting the critical nature of these proteins in maintaining immune system efficacy.