Abcam, ab174384, Anti-LexA DNA Binding Region antibody

Size: 50µL
Rabbit Polyclonal LexA antibody. Suitable for WB, ChIP, IP, ICC/IF and reacts with Escherichia coli samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Escherichia coli,
Applications:WB, ChIP, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:LexA protein of gram-negative bacteria and LexA fusion proteins for two-hybrid constructs

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-pH: 6 - 8.5Preservative: 0.05% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LexA DNA binding region is a component of the LexA protein also referred to simply as 'LexA'. LexA functions as a repressor protein in bacterial cells and has a molecular mass typically around 22.5 kDa. It is expressed in various bacterial species where it plays an important role in regulating DNA repair processes. LexA achieves its function by binding to specific DNA sequences and blocking transcription of genes involved in the SOS response.
Biological function summary
The LexA protein is critical in maintaining genomic integrity by controlling the expression of DNA repair genes. It is not usually part of a larger protein complex but operates independently within cells. In its inactive form LexA binds to the promoter regions of genes associated with the SOS response preventing their transcription. In response to DNA damage the RecA protein facilitates LexA's self-cleavage allowing for the induction of DNA repair genes.
Pathways
LexA is involved in the bacterial SOS response and DNA damage repair pathways. These pathways are vital for the organism's survival during genotoxic stress by coordinating the repair of damaged DNA. LexA works alongside other proteins such as RecA which senses DNA damage and aids in the self-cleavage of LexA releasing the suppression on SOS genes therefore initiating the repair process.
LexA's role is critical in bacterial resistance to antibiotics. The SOS response controlled by LexA can lead to increased mutation rates following antibiotic exposure potentially resulting in the development of antibiotic resistance. Understanding LexA's interactions with other proteins like RecA and its role in these processes could offer insights into more effective treatments to combat bacterial infections and reduce antibiotic resistance.