Abcam, ab1766, Anti-p55/DCAF1 antibody

Size: 100µL
Rabbit Polyclonal CAF1 antibody. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p55/DCAF1 protein also known as VprBP or HVprBP exhibits critical mechanical roles within the cell. With a mass of approximately 150 kDa this protein is a component of the DDB1–CUL4 E3 ubiquitin ligase complex. It is expressed in various tissues with significant occurrence in the brain heart and skeletal muscle. p55/DCAF1 acts as an adaptor that guides substrate proteins to the CUL4-DDB1 complex for ubiquitination influencing protein degradation and homeostasis regulation in the cell.
Biological function summary
Functions of p55/DCAF1 extend beyond mere ubiquitination facilitation. It plays a role in transcriptional regulation by interacting with nuclear receptors and histone-modifying enzymes. This protein also contributes to chromatin remodeling hinting its involvement in gene expression control. Involvement in DNA damage response adds to its biological significance connecting it with cellular repair processes. As part of a multiprotein complex p55/DCAF1 cooperates with proteins such as DDB1 in maintaining genomic integrity.
Pathways
The influence of p55/DCAF1 finds its importance in the cell cycle and DNA repair pathways. In the context of DNA damage response p55/DCAF1 works intricately with other proteins like ATR and CHK1 to monitor and respond to DNA replication stress. Additionally its participative function in the ubiquitin-proteasome pathway links it firmly to broader protein homeostasis networks where it modulates signals involving key regulators such as p53 and beta-catenin.
Researchers have found connections involving p55/DCAF1 in certain cancers and viral infections. Associations with oncogenic pathways suggest that dysregulation of this protein may contribute to tumorigenesis possibly due to its influence on cell cycle control and genomic stability. Furthermore its interaction with viral proteins such as the HIV-1 Vpr has implicated p55/DCAF1 in viral replication and pathogenesis revealing its potential as a therapeutic target in chronic viral diseases.