Abcam, ab176715, Anti-SNRNP200 antibody

Size: 100µL
Rabbit Polyclonal SNRNP200 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SNRNP200 aa 400-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SNRNP200 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.O75643

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab176715 was affinity purified using an epitope specific to SNRNP200 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNRNP200 also known as BRR2 or hBrr2 is an essential part of the spliceosome machinery. It is a large protein with a mass of approximately 250 kDa. The protein is widely expressed in various tissues with high levels in cells that have robust splicing activity. SNRNP200 is an ATPase and helicase and its mechanical role involves unwinding RNA duplexes during pre-mRNA splicing. Its activity is necessary for the rearrangements within the spliceosome facilitating the transition between different stages of the splicing cycle.
Biological function summary
SNRNP200 facilitates pre-mRNA splicing by being part of the U5 small nuclear ribonucleoprotein (snRNP) complex. The protein helps in the unwinding of RNA helices allowing snRNPs to correctly assemble and disassemble on pre-mRNA substrates. Efficient pre-mRNA splicing by SNRNP200 ensures accurate removal of introns and joining of exons leading to mature mRNA production. SNRNP200 collaborates with other spliceosomal components to maintain fidelity in the splicing process which is important for gene expression regulation.
Pathways
SNRNP200 plays integral roles in the splicing pathway and RNA processing pathway. Within the splicing pathway it interacts closely with several other spliceosomal proteins including PRPF8 and EFTUD2 to modulate spliceosome dynamics. These interactions facilitate the precise and timely execution of splicing events. The RNA processing pathway involves intricate networks of proteins where SNRNP200's helicase activity is critical for processing precursor mRNAs into mature forms for translation.
Abnormalities associated with SNRNP200 have been linked to retinitis pigmentosa and certain forms of glaucoma. Mutations in the gene encoding SNRNP200 can lead to defects in RNA splicing which in turn disrupts the expression of proteins necessary for retinal health and function. This protein also interacts with other proteins like PRPF31 and PRPF8 which are associated with similar retinal degenerative disorders. Understanding SNRNP200's role in these diseases provides insight into the molecular mechanisms underlying specific vision-related pathologies.