Abcam, ab176802, Anti-DIS3 antibody

Size: 100µL
Rabbit Polyclonal DIS3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DIS3 aa 900 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DIS3 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9Y2L1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab176802 was affinity purified using an epitope specific to DIS3 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DIS3 also known as RRP44 is an exoribonuclease and an essential component of the RNA degradation machinery. It possesses a molecular mass of approximately 110 kDa and functions as an RNA helicase and 3'-5' exoribonuclease. DIS3 is expressed in various tissues including the bone marrow and lymphoid tissues. The protein acts to degrade defective RNA molecules and regulate RNA levels ensuring accurate gene expression and proper cellular function.
Biological function summary
DIS3 participates in the RNA exosome complex a multi-protein complex responsible for processing and degrading RNA. This complex processes a wide range of RNA substrates including pre-rRNA snRNA and snoRNA. DIS3 performs its function by catalyzing the exonucleolytic cleavage of these RNA molecules regulating their abundance and contributing to RNA quality control. As a critical member of the exosome DIS3 is essential for cellular RNA homeostasis and plays a role in maintaining the stability of the transcriptome.
Pathways
RNA metabolism and degradation significantly involve DIS3 impacting mRNA surveillance and decay pathways. It notably contributes to the Nonsense-Mediated mRNA Decay (NMD) pathway which detects and degrades mRNAs containing premature stop codons. DIS3 interacts with other exosome components such as RRP6 and MTR4 facilitating the processing of diverse RNA species. The protein’s role is integral to overall RNA turnover and quality control ensuring effective removal of defective RNA transcripts and preventing inappropriate protein synthesis.
Mutations or dysregulation of DIS3 relate to multiple myeloma and acute myeloid leukemia. In these conditions aberrant RNA degradation may contribute to the pathogenesis by altering normal RNA processing and expression profiles. DIS3 mutations can lead to impaired function of the exosome enhancing the survival and proliferation of malignant cells. Additionally proteins such as RRP6 are connected with DIS3 through their roles in the exosome machinery suggesting combined involvement in RNA processing-related diseases.