Abcam, ab176819, Anti-DHX16 antibody

Size: 100µL
Rabbit Polyclonal DHX16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DHX16 aa 950 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DHX16 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O60231

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab176819 was affinity purified using an epitope specific to DHX16 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: 99% Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein DHX16 also known as DBP2 is an ATP-dependent RNA helicase belonging to the DEAH-box family. It has a molecular mass of around 130 kDa. RNA helicases like DHX16 unwind RNA structures aiding in RNA processing events. You can find DHX16 in the nucleus where it participates in the modification and maturation of pre-mRNAs.
Biological function summary
DHX16 plays a role in RNA splicing by participating in the spliceosome complex. This complex carries out the splicing of pre-mRNA introns an important step for producing mature mRNAs. By unwinding RNA structures DHX16 helps in the precise removal and rearrangement of introns and exons. The efficient function of DHX16 in the spliceosome ensures accurate genetic expression affecting various cellular functions.
Pathways
DHX16 influences the mRNA processing and splicing pathway possessing a significant relation to protein synthesis. The spliceosome where DHX16 functions acts in tandem with various other splicing factors such as SF3B1 and U2AF65 ensuring the controlled and regulated maturation of mRNA transcripts. This activity links DHX16 to gene expression pathways impacting cell development and cycle regulation.
DHX16 associates with certain genetic abnormalities and cancers. Mutations or misregulation of DHX16 can contribute to impaired RNA processing tying it to disorders such as myelodysplastic syndromes (MDS). In MDS abnormal splicing mechanisms induced by DHX16 anomalies can lead to ineffective hematopoiesis. Furthermore DHX16 might interact with proteins like SRSF2 in these conditions revealing a broader network of splicing factors involved in disease pathogenesis.