Abcam, ab176822, Anti-SART3 antibody

Size: 100µL
Rabbit Polyclonal SART3 antibody. Suitable for IP, WB, IHC-Fr and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SART3 aa 600-650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-Fr, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SART3 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.Q15020

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab176822 was affinity purified using an epitope specific to SART3 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: 99% Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SART3 also known as squamous cell carcinoma antigen recognized by T-cells 3 is a 110 kDa protein involved in various cellular processes. It is expressed in the nucleus of cells and has roles in RNA processing and splicing. SART3 assists in the assembly of the U4/U6 di-snRNP complex which is essential for the spliceosomal machinery. The protein's role in splicing indicates its participation in regulating gene expression and maintaining cellular function.
Biological function summary
SART3 plays a significant role in mRNA splicing by assisting the formation of tri-snRNP which is necessary for spliceosome activity. SART3 interacts with other splicing factors and is a component of the spliceosomal complex. Its localization in the nucleus supports its involvement in pre-mRNA splicing. This function is important for producing mature mRNA molecules which are fundamental for protein synthesis in cells.
Pathways
SART3's involvement in the spliceosome pathway highlights its importance in RNA processing. This pathway is critical for gene expression and regulation. SART3 works alongside other proteins such as U4/U6 snRNP and U5 snRNP to form and stabilize the spliceosome machinery. Another related pathway is mRNA surveillance ensuring the accuracy of mRNA processing and detecting defective transcripts. Through these pathways SART3 ensures that the cellular transcriptome is correctly processed and functional.
Aberrations in SART3 expression or function can be linked to cancer and autoimmune diseases. For instance alterations in SART3 have implications in squamous cell carcinoma where its recognized antigen role by T-cells might initiate an immune response. In autoimmune disorders abnormal splicing events linked to SART3 may contribute to disease pathogenesis. SART3 also interacts with HLA proteins in the immune system potentially influencing immune recognition and response in these disease contexts.