Abcam, ab176840, Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade

Size: 100µL / 1mL
Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (ab176840) is a rabbit monoclonal antibody detecting Histone H3.3 in Western Blot, IP, IHC-P, ICC/IF, ChIP, Dot Blot . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17899,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ChIP-seq, Dot, ICC/IF, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen sequence used for this antibody is 100% identical to the protein sequence shown for H3.X and H3.Y in Szenker et al., 2011 (PubMed ID 21263457). These H3.X and H3.Y protein sequences have not been reviewed by UniProt. The immunogen sequense is also 100% homologous to Histone H3.6. For further information on this antibody, please contact our technical support team.

Product details:
What is this antibody validated in?
Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (ab176840) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of Histone H3.3?
Anti-Histone H3.3 [EPR17899] - ChIP Grade (ab176840) specifically detects a band for Histone H3.3 (UniProt: P84243) at a molecular weight of 15kDa.
Trusted by the scientific community
Anti-Histone H3.3 [EPR17899] - ChIP Grade (ab176840) was first used in a scientific publication in 2015 and has been cited over 30 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR17899] also available for your convenience: ab176840, Carrier free -
ab208690
, Alexa Fluor® 488 -
ab313661
, Alexa Fluor® 647 -
ab313662
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle