Abcam, ab177178, Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade

Size: 20µL / 100µL / 1mL
Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP, PepArr . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 70 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP16602,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ChIP, PepArr, IHC-P, ChIP-seq, ICC/IF, ChIC/CUT&RUN-seq, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab177178 binds K27ac alone and also when S28 is phosphorylated

Product details:
Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), ICC/IF, IHC-P, PepArr, WB in human, mouse, rat samples.
Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has been cited over 78 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has 8 independent reviews from customers.
Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) specifically detects Histone H3 Acetyl-K27 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 uL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP16602 - ab32877.
Antibody clone EP16602 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 75, APC, PE (
ab245911
ab245912
ab321196
ab321197
ab321198
ab321199
ab3212
H3K27ac is a key epigenetic mark associated with gene activation and chromatin remodeling. The use of anti h3k27ac antibody and anti histone antibody can significantly enhance the detection and study of this modification. Research indicates that H3K27ac plays a crucial role in various cancers, making it a valuable target for understanding cancer progression and developing new therapeutic strategies. This antibody is also validated for ChIP-seq and CUT&RUN-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle