Abcam, ab177953, Anti-Nogo antibody [EPR12266]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal RTN4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12266,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares high homology with RTN1 (89%), RTN2 (78%), and RTN3 (78%). Cross-reactivity with RTN1, RTN2, and RTN3 has not been confirmed experimentally.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Nogo protein also known as RTN4 or Nogo-A is a member of the reticulon family with an approximate mass of 200 kDa. It is known for its inhibitory role in axonal regeneration acting as a potent neurite outgrowth inhibitor. The Nogo protein is widely expressed in the central nervous system especially in oligodendrocytes and the endoplasmic reticulum of neurons. It is an important target in neurobiology due to its involvement in regulating axonal growth.
Biological function summary
Nogo protein plays a critical role in the central nervous system by maintaining the stability of neuronal circuits and preventing excessive or aberrant plasticity. It achieves this through its association with membrane-bound complexes. The Nogo-A variant in particular interacts with the Nogo receptor (NgR1) complex to mediate its effects on axon outgrowth inhibition. This interaction is important for maintaining neural structural integrity under normal physiological conditions.
Pathways
The Nogo protein is integrated into neurite outgrowth-inhibitory pathways influencing neural connectivity. It participates mainly in the RhoA-ROCK signaling pathway a pathway known for its role in modulating the cytoskeleton and neuronal morphology. Through this pathway Nogo interacts with several proteins including the p75 neurotrophin receptor (p75^NTR) mediating downstream effects on cell shape and motility. The regulation of this pathway is significant for understanding axonal regeneration and growth inhibition.
The Nogo protein is relevant in conditions such as spinal cord injury and multiple sclerosis. In spinal cord injuries upregulated Nogo-A expression contributes to the inhibition of axonal regeneration and functional recovery. Similarly in multiple sclerosis Nogo protein's interaction with the myelin-associated inhibitors may exacerbate damage or delay in repair linked with impaired oligodendrocyte function. This connection highlights the potential therapeutic importance of targeting Nogo interactions to promote neurological recovery and repair.