Abcam, ab178847, Anti-Iba1 antibody [EPR16589] - Microglia marker

Size: 20µL / 100µL / 1mL
Anti-Iba1 antibody [EPR16589] (ab178847) is a rabbit monoclonal antibody detecting Iba1 in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 280 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16589,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-Fr, WB, IHC (PFA fixed), IHC-P, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Find all reagents to label astrocytes in our "
Microglia markers guide
Anti-Iba1 antibody [EPR16589] (ab178847) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC (PFA fixed), IHC-P, IP and WB.
Anti-Iba1 antibody [EPR16589] (ab178847) was first used in a scientific publication in 2016 and has been cited over 284 times in peer reviewed journals. It's performance in immunofluorescence and IHC in mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Iba1 antibody [EPR16589] (ab178847) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Iba1 antibody [EPR16589] (ab178847) has 18 independent reviews from customers.
Anti-Iba1 antibody [EPR16589] (ab178847) specifically detects Iba1 (UniProt ID: P55008; Molecular weight: 17kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR16589 -
ab221790
Antibody clone EPR16589 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
594, Alexa Fluor
488, Alexa Fluor
568, Alexa Fluor
555, Alexa Fluor
ab216544
ab310987
ab311112
ab311734
ab312913
ab313011
ab313215
ab321219
NEW: Explore our alternative host species for clone [EPR16589] : rat, mouse. Iba1 (AIF1) is a marker for microglia, the brain's primary immune cells and is frequently used in Alzheimer's disease (AD) research. In AD brains, altered Iba1 expression indicates microglial activation and neuroinflammation. Researchers use Iba1 to assess microglial response and motility in AD models, making it essential for understanding the role of inflammation and immune response in Alzheimer's pathology.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
Biological function summary
Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.
Pathways
Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.
Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.