Abcam, ab179800, Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]

Size: 20µL / 100µL / 1mL
Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) is a rabbit monoclonal antibody detecting COX2 / Cyclooxygenase 2 in Western Blot, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 120 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12012,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Stimulation is required to allow detection of the COX2 protein in some cell lines and tissues. It is better to use a positive control side by side when testing.Rat species is recommended based on IHC result, we do not guarantee WB, IP and ICC/IF for Rat.For western blot application, this antibody has low sensitivity issue. We suggest optimizing experimental protocols (increasing lysate amount, using lower antibody dilution or higher sensitivity ECL substrate) to improve results. We also recommend ab283574 as an alternative for testing COX2 in western blot.

Product details:
Product Specifications
Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) specifically detects COX2 / Cyclooxygenase 2 (UniProt ID: P35354; Molecular weight: 67kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) has been confirmed by testing in knockout samples.
Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) has been cited over 127 times in peer reviewed journals and is trusted by the scientific community.
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Target Information
Cyclooxygenase-2 (COX-2) is an enzyme crucial for the inflammatory response, as it converts arachidonic acid into prostaglandins that mediate inflammation and pain. It is involved in various diseases, such as cancer, cardiovascular diseases, and neurodegenerative disorders, due to its role in promoting inflammation and tumor growth.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cyclooxygenase 2 also known as COX2 is an enzyme involved in the conversion of arachidonic acid to prostaglandins which are lipid compounds with hormone-like effects. It has alternative names including prostaglandin-endoperoxide synthase 2. The molecular weight of COX2 is approximately 72 kDa. This enzyme is expressed in various tissues including the brain kidneys and areas of inflammation. COX2 expression increases during inflammatory responses and is induced by pro-inflammatory cytokines.
Biological function summary
COX2 plays a significant role in the inflammatory response and is part of the complex process of synthesizing prostaglandins. These compounds mediate inflammation and pain making COX2 an important target for understanding these processes. COX2 is not ubiquitously expressed but rather is induced in activated macrophages and other cells during inflammatory conditions. Its function is also important for normal physiological processes like ovulation and implantation.
Pathways
COX2 is essential in the prostaglandin biosynthesis pathway connecting it to the arachidonic acid metabolism pathway. Cyclooxygenase 2 works with phospholipase A2 which releases arachidonic acid from the phospholipid membrane. COX2 then converts this acid to prostaglandin H2 a precursor for other prostaglandins. COX1 the other isoform of cyclooxygenase is closely related to COX2 and while they have different expression patterns they share some functional similarities in these pathways.
COX2 is connected to inflammatory conditions like arthritis and cancer. Its expression often increases in various cancer types contributing to tumor growth and metastasis by promoting angiogenesis and suppressing immune responses. The enzyme is also linked to rheumatoid arthritis where its overexpression exacerbates inflammation. COX2 inhibitors like ketorolac tromethamine or naproxen structure mitigate symptoms by decreasing prostaglandin synthesis. These inhibitors also interact with COX1 but selective inhibition of COX2 targets inflammation more effectively with fewer gastric side effects associated with COX1 inhibition.