Abcam, ab180177, Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Tripeptidyl peptidase II/TPPII antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13202,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt (Intra), ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tripeptidyl peptidase II (TPPII) also known as TPP2 is a large peptidase complex that mechanistically degrades oligopeptides into tripeptides. It consists of a massive molecular complex with a molecular mass of approximately 5-6 MDa. TPPII is ubiquitously expressed in various tissues with high expression levels in the liver muscle and kidney. This complex is cytosolic and possesses unique endopeptidase and exopeptidase activities which makes it distinct in both structure and function from the proteasome.
Biological function summary
TPPII is an enzyme that plays a vital role in the regulation of intracellular protein turnover and antigen processing. It contributes to protein degradation especially when proteasome activities are inhibited or overwhelmed. TPPII operates in a complex and involves the dynamic assembly of its subunits to form a functional peptidase complex. Through its action TPPII maintains protein homeostasis and assists in generating peptides presented by MHC class I molecules important for immune surveillance.
Pathways
TPPII functions within the ubiquitin-proteasome system and the antigen presentation pathway. It acts as a backup to the proteasome ensuring continued protein degradation. TPPII interacts with proteins such as ubiquitin and E3 ligases which tag proteins for degradation. During antigen processing TPPII complements proteasome activity by processing longer peptides into shorter ones suitable for MHC class I presentation thereby interfacing with molecules like TAP and the proteasome subunits.
Dysregulation of TPPII is associated with conditions such as cancer and neurodegenerative diseases. The upregulation of TPPII has been observed in several tumors implicating its role in promoting cell survival and proliferation. In cancer TPPII may interact with proteins such as p53 affecting apoptosis and cell cycle regulation. In neurodegenerative diseases altered TPPII function might influence protein aggregation and degradation pathways where it might relate with proteins like tau and amyloid-beta contributing to disease progression.