Abcam, ab180332, Recombinant Human GTSF1 protein

Size: 100µg
Recombinant Human GTSF1 protein is a Human Full Length protein, in the 1 to 167 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q8WW33,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GTSF1 also known as gametocyte specific factor 1 functions mechanically in the regulation of gene expression particularly in the germ cells. It is a protein with a molecular mass of approximately 29 kDa. GTSF1 expresses mainly in the testes and ovaries suggesting its role in reproductive biology. This target's interaction with nucleic acids is an important part of its function as it plays a role in maintaining genomic stability during gametogenesis.
Biological function summary
In the context of germ cell development GTSF1 interacts with the Piwi-interacting RNA (piRNA) pathway. It is part of a larger protein complex that influences RNA silencing playing a significant role in repressing transposable elements. This activity helps protect the integrity of the genome by preventing harmful genetic instability. The protein complex involving GTSF1 is important for the correct progression of gametogenesis impacting fertility and reproductive success.
Pathways
The involvement of GTSF1 in the piRNA pathway is essential for the silencing mechanism that targets transposable elements. This pathway is integral to maintaining germ cell genome integrity. GTSF1 works alongside other proteins like PIWI which aids in assembling the piRNA-induced silencing complex (piRISC). Through this involvement GTSF1 contributes to a downstream effect on various biosynthesis and protective mechanisms that ensure proper cell cycle progression.
Disrupted GTSF1 function is linked to reproductive health issues including infertility. Defects in piRNA pathway components related to GTSF1 failure may result in germ cell apoptosis or suboptimal gamete production. Furthermore malfunction in GTSF1 interactions could relate to gynecological disorders. GTSF1's interaction with PIWI proteins also ties it to cancer as abnormalities in piRNA pathways are sometimes observed in tumorigenesis due to their role in genomic stability.